Elaboration of Materials with Functionality Gradients by Assembly of Chitosan-Collagen Microspheres Produced by Microfluidics

Abstract : Biopolymers extracted from renewable resources like chitosan and collagen exhibit interesting properties for the elaboration of materials designed for tissue engineering applications, among which are their hydrophilicity, biocompatibility and biodegradability. In many cases, functional recovery of an injured tissue or organ requires oriented cell outgrowth, which is particularly critical for nerve regeneration. Therefore, there is a growing interest for the elaboration of materials exhibiting functionalization gradients able to guide cells. Here, we explore an original way of elaborating such gradients by assembling particles from a library of functionalized microspheres. We propose a simple process to prepare chitosan-collagen hybrid microspheres by micro- and milli-fluidics, with adaptable dimensions and narrow size distributions. The adhesion and survival rate of PC12 cells on hybrid microspheres were compared to those on pure chitosan ones. Finally, functionalized microspheres were assembled into membranes exhibiting a functionalization gradient.
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https://hal-cea.archives-ouvertes.fr/cea-01695847
Contributeur : Serge Palacin <>
Soumis le : lundi 29 janvier 2018 - 18:15:05
Dernière modification le : jeudi 15 mars 2018 - 15:04:08

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David Azria, Raluca Guermache, Sophie Raisin, Sébastien Blanquer, Frédéric Gobeaux, et al.. Elaboration of Materials with Functionality Gradients by Assembly of Chitosan-Collagen Microspheres Produced by Microfluidics. Journal of Renewable Materials, Scrivener publishing, 2018, 〈http://www.ingentaconnect.com/content/scrivener/jrm/pre-prints/content-jrm-2017-0056#Supp〉. 〈10.7569/JRM.2017.634186〉. 〈cea-01695847〉

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