Manipulating hemoglobin oxygenation using silica nanoparticles: a novel prospect for artificial oxygen carriers

Stephanie Devineau 1 Laurent Kiger 2 Frédéric Galacteros 2 Véronique Baudin-Creuza 2 Michael Marden 2 Jean Philippe Renault 1 Serge Pin 1
1 LIONS - Laboratoire Interdisciplinaire sur l'Organisation Nanométrique et Supramoléculaire
NIMBE UMR 3685 - Nanosciences et Innovation pour les Matériaux, la Biomédecine et l'Energie (ex SIS2M)
2 IMRB - Institut Mondor de Recherche Biomédicale
Abstract : Silica nanoparticles act as an effector for human native and sickle cell hemoglobin while preserving their tetrameric structure.Manipulating hemoglobin oxygenation using nanoparticles opens the way for the rational design of hemoglobin-based oxygen carriers.Recently, nanoparticles have attracted much attention as new scaffolds for hemoglobin-based oxygen carriers (HBOCs). Indeed, the development of bionanotechnology paves the way for the rational design of blood substitutes, providing that the interaction between the nanoparticles and hemoglobin at a molecular scale and its effect on the oxygenation properties of hemoglobin are finely controlled. Here, we show that human hemoglobin has a high affinity for silica nanoparticles, leading to the adsorption of hemoglobin tetramers on the surface. The adsorption process results in a remarkable retaining of the oxygenation properties of human adult hemoglobin and sickle cell hemoglobin, associated with an increase of the oxygen affinity. The cooperative oxygen binding exhibited by adsorbed hemoglobin and the comparison with the oxygenation properties of diaspirin cross-linked hemoglobin confirmed the preservation of the tetrameric structure of hemoglobin loaded on silica nanoparticles. Our results show that silica nanoparticles can act as an effector for human native and mutant hemoglobin. Manipulating hemoglobin oxygenation using nanoparticles opens the way to the design of novel HBOCs.
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Article dans une revue
Blood Advances, The American Society of Hematology, 2018, 2 (2), pp.90 - 94. 〈10.1182/bloodadvances.2017012153〉
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Contributeur : Serge Palacin <>
Soumis le : lundi 29 janvier 2018 - 16:12:14
Dernière modification le : jeudi 15 mars 2018 - 15:04:08

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Stephanie Devineau, Laurent Kiger, Frédéric Galacteros, Véronique Baudin-Creuza, Michael Marden, et al.. Manipulating hemoglobin oxygenation using silica nanoparticles: a novel prospect for artificial oxygen carriers. Blood Advances, The American Society of Hematology, 2018, 2 (2), pp.90 - 94. 〈10.1182/bloodadvances.2017012153〉. 〈cea-01695665〉

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