Synthesis of Mono- and Dihydroxylated Amino Acids with New $\alpha$-Ketoglutarate-Dependent Dioxygenases: Biocatalytic Oxidation of C$-$H Bonds

Abstract : Iron(II)/$\alpha$-ketoacid-dependent oxygenases ($\alpha$KAOs) are enzymes that mainly catalyse hydroxylation reaction. By using a genomic approach combining sequence comparison and protein-domain sharing, a set of 131 $\alpha$KAO enzymes was prepared. The screening of various substrates revealed five new $\alpha$KAOs. Four $\alpha$KAOs were found to be active towards L-lysine, L-ornithine and L-arginine with total regio- and stereoselectivities and yielding the corresponding 3- or 4-hydroxyamino acids. The enzymatic cascade reaction with two stereoselective regiodivergent $\alpha$ KAOs enabled the synthesis of 3,4-dihydroxy-L- lysine.
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ChemCatChem, Wiley, 2014, 6, pp.3012 - 3017. 〈10.1002/cctc.201402498〉
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Damien Baud, Pierre-Loïc Saaidi, Adam Monfleur, Marine Harari, Julien Cuccaro, et al.. Synthesis of Mono- and Dihydroxylated Amino Acids with New $\alpha$-Ketoglutarate-Dependent Dioxygenases: Biocatalytic Oxidation of C$-$H Bonds. ChemCatChem, Wiley, 2014, 6, pp.3012 - 3017. 〈10.1002/cctc.201402498〉. 〈cea-01591870〉

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