Synthesis of Mono- and Dihydroxylated Amino Acids with New $\alpha$-Ketoglutarate-Dependent Dioxygenases: Biocatalytic Oxidation of C$-$H Bonds
Résumé
Iron(II)/$\alpha$-ketoacid-dependent oxygenases ($\alpha$KAOs) are enzymes
that mainly catalyse hydroxylation reaction. By using
a genomic approach combining sequence comparison and
protein-domain sharing, a set of 131 $\alpha$KAO enzymes was prepared.
The screening of various substrates revealed five new
$\alpha$KAOs. Four $\alpha$KAOs were found to be active towards L-lysine,
L-ornithine and L-arginine with total regio- and stereoselectivities
and yielding the corresponding 3- or 4-hydroxyamino
acids. The enzymatic cascade reaction with two stereoselective
regiodivergent $\alpha$ KAOs enabled the synthesis of 3,4-dihydroxy-L-
lysine.
Domaines
Catalyse
Origine : Fichiers produits par l'(les) auteur(s)
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