Synthesis of Mono- and Dihydroxylated Amino Acids with New $\alpha$-Ketoglutarate-Dependent Dioxygenases: Biocatalytic Oxidation of C$-$H Bonds - CEA - Commissariat à l’énergie atomique et aux énergies alternatives Access content directly
Journal Articles ChemCatChem Year : 2014

Synthesis of Mono- and Dihydroxylated Amino Acids with New $\alpha$-Ketoglutarate-Dependent Dioxygenases: Biocatalytic Oxidation of C$-$H Bonds

Abstract

Iron(II)/$\alpha$-ketoacid-dependent oxygenases ($\alpha$KAOs) are enzymes that mainly catalyse hydroxylation reaction. By using a genomic approach combining sequence comparison and protein-domain sharing, a set of 131 $\alpha$KAO enzymes was prepared. The screening of various substrates revealed five new $\alpha$KAOs. Four $\alpha$KAOs were found to be active towards L-lysine, L-ornithine and L-arginine with total regio- and stereoselectivities and yielding the corresponding 3- or 4-hydroxyamino acids. The enzymatic cascade reaction with two stereoselective regiodivergent $\alpha$ KAOs enabled the synthesis of 3,4-dihydroxy-L- lysine.

Domains

Catalysis
Fichier principal
Vignette du fichier
Baud_et_al-2014-ChemCatChem.pdf (295.83 Ko) Télécharger le fichier
Origin : Files produced by the author(s)
Loading...

Dates and versions

cea-01591870 , version 1 (22-09-2017)

Identifiers

Cite

Damien Baud, Pierre-Loïc Saaidi, Adam Monfleur, Marine Harari, Julien Cuccaro, et al.. Synthesis of Mono- and Dihydroxylated Amino Acids with New $\alpha$-Ketoglutarate-Dependent Dioxygenases: Biocatalytic Oxidation of C$-$H Bonds. ChemCatChem, 2014, 6, pp.3012 - 3017. ⟨10.1002/cctc.201402498⟩. ⟨cea-01591870⟩
363 View
424 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More