DMF-MALDI: droplet based microfluidic combined to MALDI-TOF for focused peptide detection

Abstract : We present an automated droplet microfluidic system (DMF) to generate monitored nanoliter aqueous droplets in oil and their deposition on a commercial stainless steel plate for MALDI-TOF analysis of peptides or protein digests. We demonstrate that DMF-MALDI combination focuses the analyte on the MALDI plate, increasing considerably the homogeneity of the dried material. This results in a 30times enhanced MALDI-TOF MS signal for a model peptide, allowing a significant improvement of the detection sensitivity limit (down to few tens of attomoles). Moreover, positive detection can be achieved from sub-nanomolar peptides solutions and better overall protein sequence coverages are obtained from few tens attomoles of protein digest. These results make DMF-MALDI a promising approach for the treatment of peptides samples as well as a key component for an integrated approach in the proteomic field.
Type de document :
Article dans une revue
Scientific Reports, Nature Publishing Group, 2017, 7 (1), pp.6756. 〈https://doi.org/10.1038/s41598-017-06660-8〉. 〈10.1038/s41598-017-06660-8〉
Domaine :
Liste complète des métadonnées

Littérature citée [31 références]  Voir  Masquer  Télécharger

https://hal-cea.archives-ouvertes.fr/cea-01570543
Contributeur : Serge Palacin <>
Soumis le : lundi 31 juillet 2017 - 09:46:47
Dernière modification le : vendredi 30 mars 2018 - 01:16:36

Fichier

s41598-017-06660-8.pdf
Fichiers éditeurs autorisés sur une archive ouverte

Identifiants

Citation

Kiarach Mesbah, Robert Thai, Sarah Bregant, Florent Malloggi. DMF-MALDI: droplet based microfluidic combined to MALDI-TOF for focused peptide detection. Scientific Reports, Nature Publishing Group, 2017, 7 (1), pp.6756. 〈https://doi.org/10.1038/s41598-017-06660-8〉. 〈10.1038/s41598-017-06660-8〉. 〈cea-01570543〉

Partager

Métriques

Consultations de la notice

108

Téléchargements de fichiers

45