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DMF-MALDI: droplet based microfluidic combined to MALDI-TOF for focused peptide detection

Abstract : We present an automated droplet microfluidic system (DMF) to generate monitored nanoliter aqueous droplets in oil and their deposition on a commercial stainless steel plate for MALDI-TOF analysis of peptides or protein digests. We demonstrate that DMF-MALDI combination focuses the analyte on the MALDI plate, increasing considerably the homogeneity of the dried material. This results in a 30times enhanced MALDI-TOF MS signal for a model peptide, allowing a significant improvement of the detection sensitivity limit (down to few tens of attomoles). Moreover, positive detection can be achieved from sub-nanomolar peptides solutions and better overall protein sequence coverages are obtained from few tens attomoles of protein digest. These results make DMF-MALDI a promising approach for the treatment of peptides samples as well as a key component for an integrated approach in the proteomic field.
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Submitted on : Monday, July 31, 2017 - 9:46:47 AM
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Kiarach Mesbah, Robert Thai, Sarah Bregant, Florent Malloggi. DMF-MALDI: droplet based microfluidic combined to MALDI-TOF for focused peptide detection. Scientific Reports, Nature Publishing Group, 2017, 7 (1), pp.6756. ⟨10.1038/s41598-017-06660-8⟩. ⟨cea-01570543⟩

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