A Highly Selective Potassium Sensor for the Detection of Potassium in Living Tissues

Alexis Depauw; Elena Dossi; Naresh Kumar; Céline Fiorini-Debuisschert; Gilles Huberfeld; Minh-Huong Ha-Thi; Nathalie Rouach; Isabelle Leray

doi:10.1002/chem.201602209

A Highly Selective Potassium Sensor for the Detection of Potassium in Living Tissues

Alexis Depauw ¹ Elena Dossi Naresh Kumar ² Céline Fiorini-Debuisschert ³ Gilles Huberfeld ⁴ Minh-Huong Ha-Thi ⁵ Nathalie Rouach ⁶ Isabelle Leray ¹

1 PPSM - Laboratoire de Photophysique et Photochimie Supramoléculaires et Macromoléculaires

2 National Institute of Technology, Jamshedpur

3 LEPO - Laboratoire d'Electronique et nanoPhotonique Organique

SPEC - UMR3680 - Service de physique de l'état condensé, IRAMIS - Institut Rayonnement Matière de Saclay

4 U1129 - Epilepsies de l'Enfant et Plasticité Cérébrale

5 ISMO - Institut des Sciences Moléculaires d'Orsay

6 CIRB - Centre interdisciplinaire de recherche en biologie

Abstract : The development of highly selective sensors for potassium is of great interest in biology. Two new hydrosoluble potassium sensors (Calix-COU-Alkyne and Calix-COU-Am) based on a calix[4]arene bis(crown-6) and an extended coumarin were synthesized and characterized. The photophysical properties and complexation studies of these compounds have been investigated and show high molar extinction coefficients and high fluorescence quantum yields. Upon complexation with potassium in the millimolar concentration range, an increase of one- and two-photon fluorescence emission is detected. A twofold fluorescence enhancement is observed upon excitation at λ=405 nm. The ligands present excellent selectivity for potassium in the presence of various competitive cations in water and in a physiological medium. The photophysical properties are not affected by the presence of a large amount of competing cations (Na+ , Ca2+ , Mg2+ , etc.). Ex vivo measurements on mouse hippocampal slices show that Calix-COU-Alkyne accumulates extracellularly and does not alter the neuronal activity. Furthermore, the sensor can be utilized to monitor slow extracellular K+ increase induced by inhibition of K+ entry into the cells.

Type de document :

Article dans une revue

Chemistry - A European Journal, Wiley-VCH Verlag, 2016, 22 (42), pp.14902 - 14911. 〈10.1002/chem.201602209〉

Domaine :

Physique [physics]

Liste complète des métadonnées

https://hal-cea.archives-ouvertes.fr/cea-01485211
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Dernière modification le : jeudi 7 février 2019 - 17:12:41

A Highly Selective Potassium Sensor for the Detection of Potassium in Living Tissues

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