A Highly Selective Potassium Sensor for the Detection of Potassium in Living Tissues - CEA - Commissariat à l’énergie atomique et aux énergies alternatives Access content directly
Journal Articles Chemistry - A European Journal Year : 2016

A Highly Selective Potassium Sensor for the Detection of Potassium in Living Tissues

Abstract

The development of highly selective sensors for potassium is of great interest in biology. Two new hydrosoluble potassium sensors (Calix-COU-Alkyne and Calix-COU-Am) based on a calix[4]arene bis(crown-6) and an extended coumarin were synthesized and characterized. The photophysical properties and complexation studies of these compounds have been investigated and show high molar extinction coefficients and high fluorescence quantum yields. Upon complexation with potassium in the millimolar concentration range, an increase of one- and two-photon fluorescence emission is detected. A twofold fluorescence enhancement is observed upon excitation at λ=405 nm. The ligands present excellent selectivity for potassium in the presence of various competitive cations in water and in a physiological medium. The photophysical properties are not affected by the presence of a large amount of competing cations (Na+ , Ca2+ , Mg2+ , etc.). Ex vivo measurements on mouse hippocampal slices show that Calix-COU-Alkyne accumulates extracellularly and does not alter the neuronal activity. Furthermore, the sensor can be utilized to monitor slow extracellular K+ increase induced by inhibition of K+ entry into the cells.

Dates and versions

cea-01485211 , version 1 (08-03-2017)

Identifiers

Cite

Alexis Depauw, Elena Dossi, Naresh Kumar, Céline Fiorini-Debuisschert, Gilles Huberfeld, et al.. A Highly Selective Potassium Sensor for the Detection of Potassium in Living Tissues. Chemistry - A European Journal, 2016, 22 (42), pp.14902 - 14911. ⟨10.1002/chem.201602209⟩. ⟨cea-01485211⟩
150 View
0 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More