High Sensitivity High-Resolution Full Range Relaxometry Using a Fast Mechanical Sample Shuttling Device and a Cryo-probe

Ching-Yu Chou (1, 2, 3) , Minglee Chu (4) , Chi-Fon Chang (5) , Tsunai Yu (6) , Tai-Huang Huang (6) , Dimitris Sakellariou (2)

1 UPMC - Université Pierre et Marie Curie - Paris 6
2 LSDRM - Laboratoire Structure et Dynamique par Résonance Magnétique (LCF)
3 FCT - Field Cycling Technology Ltd.
4 Institute of Physics [Taipei]
5 Genomics Research Center
6 Institute of Biomedical Science

Field-dependent NMR studies of bio-molecular systems using a sample shuttling hardware operating on a high-field NMR apparatus have provided valuable structural and dynamic information. We have recently published a design of a compact sample transportation device, called ‘‘field-cycler’’, which was installed in a commercial spectrometer and which provided highly precise positioning and stability during high speed shuttling. In this communication, we demonstrate the first use of a sample shuttling device on a commercial high field standard bore NMR spectrometer, equipped with a commercial triple resonance cryogenically cooled NMR probe. The performance and robustness of the hardware operating in 1D and 2D field cycling experiments, as well as the impact of the sample shuttling time on the signal intensity are discussed.

Domains: Material chemistry

Ching-Yu Chou, Minglee Chu, Chi-Fon Chang, Tsunai Yu, Tai-Huang Huang, et al.. High Sensitivity High-Resolution Full Range Relaxometry Using a Fast Mechanical Sample Shuttling Device and a Cryo-probe. Journal of Biomolecular NMR, 2016, pp.1-8. ⟨10.1007/s10858-016-0066-5⟩. ⟨cea-01383405⟩