Evaluation of a high-resolution micro-sized magic angle spinning (HR[small mu ]MAS) probe for NMR-based metabolomic studies of nanoliter samples

Tuan Nghia Duong (1, 2) , Yuki Endo (2) , Takahiro Nemoto (2) , Hiroshi Kato (2) , Anne-Karine Bouzier-Sore (3) , Yusuke Nishiyama (1, 2) , Alan Wong (4)

1 RIKEN CLST - RIKEN Center for Life Science Technologies
2 JEOL Ltd, 1156 Nakagami, Akishima, Tokyo 196-0022, Japan.
3 CRMSB - Centre de résonance magnétique des systèmes biologiques
4 LSDRM - Laboratoire Structure et Dynamique par Résonance Magnétique (LCF)

This study evaluates the performances of a recently introduced 1 mm HR[small mu ]MAS NMR probe for metabolic profiling of nanoliter samples. It examines essential NMR criteria such as spectral data qualities and repeatability, and experimental practicality. The report also discusses the difficulties related to the sample preparation in HR[small mu ]MAS experiments and considers possible solutions and improvements.

Domains: Chemical Sciences; Material chemistry

Tuan Nghia Duong, Yuki Endo, Takahiro Nemoto, Hiroshi Kato, Anne-Karine Bouzier-Sore, et al.. Evaluation of a high-resolution micro-sized magic angle spinning (HR[small mu ]MAS) probe for NMR-based metabolomic studies of nanoliter samples. Analytical Methods, 2016, 8, pp.6815-6820. ⟨10.1039/C6AY01654E⟩. ⟨cea-01380672⟩