Implementation of laser induced fluorescence in a pulse radiolysis experiment – a new way to analyze resazurin-like reduction mechanisms

A. Balcerzyk; G. Baldacchino

Implementation of laser induced fluorescence in a pulse radiolysis experiment – a new way to analyze resazurin-like reduction mechanisms

A. Balcerzyk ¹ G. Baldacchino ^{2, 3}

1 LIONS - Laboratoire Interdisciplinaire sur l'Organisation Nanométrique et Supramoléculaire

NIMBE UMR 3685 - Nanosciences et Innovation pour les Matériaux, la Biomédecine et l'Energie (ex SIS2M)

2 DICO - Biomolécules Excitées

IRAMIS - Institut Rayonnement Matière de Saclay, LIDyl - Laboratoire Interactions, Dynamique et Lasers (ex SPAM)

3 LIDyl - Laboratoire Interactions, Dynamique et Lasers (ex SPAM)

Abstract : Resazurin (RNO) reduction by hydrated electrons produces a fluorescent molecule: resorufin (RN). To take advantage of RN fluorescence, a novel setup is designed by implementing fluorescence detection induced by laser in a pulse radiolysis experiment. Time resolved fluorescence spectra were recorded with a fast gated intensified CCD camera during the reduction of RNO from μs to ms. Two 532 nm laser types have been used to describe the short μs range by a 5 ns Q-switch laser and the μs–ms range by a CW DPSS laser. By fitting the simulated model to the experimental data a second order rate constant of 109 M−1 s−1 was re-evaluated. This method should be considered in the near future in many in situ and real time measurements for evaluating radical production.

Document type :

Journal articles

Domain :

Chemical Sciences / Theoretical and/or physical chemistry

Complete list of metadatas

https://hal-cea.archives-ouvertes.fr/cea-01296723
Contributor : Caroline Lebe <>
Submitted on : Friday, April 1, 2016 - 2:31:34 PM
Last modification on : Thursday, February 7, 2019 - 5:10:58 PM

Implementation of laser induced fluorescence in a pulse radiolysis experiment – a new way to analyze resazurin-like reduction mechanisms

Identifiers

Collections

Citation

Export

Metrics

86

Contact

Implementation of laser induced fluorescence in a pulse radiolysis experiment – a new way to analyze resazurin-like reduction mechanisms

Identifiers

Collections

Citation

Export

Share

Metrics

86

Contact