Implementation of laser induced fluorescence in a pulse radiolysis experiment – a new way to analyze resazurin-like reduction mechanisms

A. Balcerzyk 1 G. Baldacchino 2, 3
1 LIONS - Laboratoire Interdisciplinaire sur l'Organisation Nanométrique et Supramoléculaire
NIMBE UMR 3685 - Nanosciences et Innovation pour les Matériaux, la Biomédecine et l'Energie (ex SIS2M)
2 DICO - Biomolécules Excitées
IRAMIS - Institut Rayonnement Matière de Saclay, LIDyl - Laboratoire Interactions, Dynamiques et Lasers (ex SPAM)
3 LIDyl - Laboratoire Interactions, Dynamiques et Lasers (ex SPAM)
Abstract : Resazurin (RNO) reduction by hydrated electrons produces a fluorescent molecule: resorufin (RN). To take advantage of RN fluorescence, a novel setup is designed by implementing fluorescence detection induced by laser in a pulse radiolysis experiment. Time resolved fluorescence spectra were recorded with a fast gated intensified CCD camera during the reduction of RNO from μs to ms. Two 532 nm laser types have been used to describe the short μs range by a 5 ns Q-switch laser and the μs–ms range by a CW DPSS laser. By fitting the simulated model to the experimental data a second order rate constant of 109 M−1 s−1 was re-evaluated. This method should be considered in the near future in many in situ and real time measurements for evaluating radical production.
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Journal articles
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https://hal-cea.archives-ouvertes.fr/cea-01296723
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Submitted on : Friday, April 1, 2016 - 2:31:34 PM
Last modification on : Monday, September 2, 2019 - 5:32:02 PM

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CEA | DSM-IRAMIS | IRAMIS-NIMBE | CNRS | IRAMIS-LIDYL | INC-CNRS | CEA-DRF

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A. Balcerzyk, G. Baldacchino. Implementation of laser induced fluorescence in a pulse radiolysis experiment – a new way to analyze resazurin-like reduction mechanisms . Analysis, Oldenbourg Verlag, 2014, 139, pp.1707-1712. ⟨10.1039/C3AN02000B⟩. ⟨cea-01296723⟩

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