Fast ferrous heme-NO oxidation in nitric oxide synthases.

Abstract : During catalysis, the heme in nitric oxide synthase (NOS) binds NO before releasing it to the environment. Oxidation of the NOS ferrous heme-NO complex by O2 is key for catalytic cycling, but the mechanism is unclear. We utilized stopped-flow methods to study the reaction of O2 with ferrous heme-NO complexes of inducible and neuronal NOS enzymes. We found that the reaction does not involve heme-NO dissociation, but instead proceeds by a rapid direct reaction of O2 with the ferrous heme-NO complex. This behavior is novel and may distinguish heme-thiolate enzymes, such as NOS, from related heme proteins.
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Jesús Tejero, J. Santolini, Dennis J Stuehr. Fast ferrous heme-NO oxidation in nitric oxide synthases.. FEBS Journal, Wiley, 2009, 276 (16), pp.4505-14. ⟨10.1111/j.1742-4658.2009.07157.x⟩. ⟨cea-01234308⟩

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