Atomic view of the histidine environment stabilizing higher-pH conformations of pH-dependent proteins

Céline Valéry; Stéphanie Deville-Foillard; Christelle Lefebvre; Nuria Taberner; Pierre Legrand; Florian Meneau; Cristelle Mériadec; Camille Delvaux; Thomas Bizien; Emmanouil Kasotakis; Carmen Lopez-Iglesias; Andrew Gall; Stéphane Bressanelli; Marie-Hélène Le Du; Maıté Paternostre; Franck Artzner

Atomic view of the histidine environment stabilizing higher-pH conformations of pH-dependent proteins

Céline Valéry ^{1, 2} Stéphanie Deville-Foillard ^{3, 4} Christelle Lefebvre ⁵ Nuria Taberner ¹ Pierre Legrand ⁶ Florian Meneau ⁶ Cristelle Mériadec ⁵ Camille Delvaux ^{4, 3} Thomas Bizien ⁵ Emmanouil Kasotakis ^{4, 3} Carmen Lopez-Iglesias ⁷ Andrew Gall ^{4, 3} Stéphane Bressanelli ^{8, 4} Marie-Hélène Le Du ^{9, 4} Maıté Paternostre ^{4, 8} Franck Artzner ⁵

1 IPSEN

2 Biomolecular Interaction Centre

3 I2BC - Institut de Biologie Intégrative de la Cellule

4 IBITECS - Institut de Biologie et de Technologies de Saclay

5 IPR - Institut de Physique de Rennes

6 SSOLEIL - Synchrotron SOLEIL

7 Cryo-Electron Microscopy Unit, Scientific and Tecnological Centers

8 IMAPP - Interactions et mécanismes d’assemblage des protéines et des peptides

B3S - Département Biochimie, Biophysique et Biologie Structurale

9 INTGEN - Enveloppe Nucléaire, Télomères et Réparation de l’ADN

B3S - Département Biochimie, Biophysique et Biologie Structurale

Abstract : External stimuli are powerful tools that naturally control protein assemblies and functions. For example, during viral entry and exit changes in pH are known to trigger large protein conformational changes. However, the molecular features stabilizing the higher pH structures remain unclear. Here we elucidate the conformational change of a self-assembling peptide that forms either small or large nanotubes dependent on the pH. The sub-angstrom high-pH peptide structure reveals a globular conformation stabilized through a strong histidine-serine H-bond and a tight histidine-aromatic packing. Lowering the pH induces histidine protonation, disrupts these interactions and triggers a large change to an extended b-sheet-based conformation. Re-visiting available structures of proteins with pH-dependent conformations reveals both histidine-containing aromatic pockets and histidine-serine proximity as key motifs in higher pH structures. The mechanism discovered in this study may thus be generally used by pH-dependent proteins and opens new prospects in the field of nanomaterials.

Document type :

Journal articles

Domain :

Life Sciences [q-bio]

Physics [physics]

Complete list of metadatas

Cited literature [39 references] Display Hide Download

https://hal-cea.archives-ouvertes.fr/cea-01201915
Contributor : Magali Le Discorde <>
Submitted on : Friday, September 18, 2015 - 12:28:50 PM
Last modification on : Thursday, July 18, 2019 - 2:54:12 PM
Long-term archiving on : Tuesday, December 29, 2015 - 8:39:51 AM

36813.pdf

Publication funded by an institution

Atomic view of the histidine environment stabilizing higher-pH conformations of pH-dependent proteins

File

Identifiers

Collections

Citation

Export

Metrics

720

178

Contact

Atomic view of the histidine environment stabilizing higher-pH conformations of pH-dependent proteins

File

Identifiers

Collections

Citation

Export

Share

Metrics

720

178

Contact