E. Cascales and P. J. Christie, The versatile bacterial type IV secretion systems, Nat Rev Microbiol, vol.1, pp.137-149, 2003.

P. J. Christie, K. Atmakuri, V. Krishnamoorthy, S. Jakubowski, and E. Cascales, Biogenesis, architecture, and function of bacterial type IV secretion systems, Annu Rev Microbiol, vol.59, pp.451-485, 2005.

G. Schroder and E. Lanka, The mating pair formation system of conjugative plasmids-A versatile secretion machinery for transfer of proteins and DNA, Plasmid, vol.54, pp.1-25, 2005.

S. Backert and M. Selbach, Role of type IV secretion in Helicobacter pylori pathogenesis, Cell Microbiol, vol.10, pp.1573-1581, 2008.

S. Ninio and C. R. Roy, Effector proteins translocated by Legionella pneumophila: strength in numbers, Trends Microbiol, vol.15, pp.372-380, 2007.

C. A. Mccullen and A. N. Binns, Agrobacterium tumefaciens and plant cell interactions and activities required for interkingdom macromolecular transfer, Annu Rev Cell Dev Biol, vol.22, pp.101-127, 2006.

D. L. Burns, Type IV transporters of pathogenic bacteria, Curr Opin Microbiol, vol.6, pp.29-34, 2003.

C. M. Thomas and K. M. Nielsen, Mechanisms of, and Barriers to, Horizontal Gene Transfer between Bacteria, Nat Rev Micro, vol.3, pp.711-721, 2005.

R. Fronzes, H. Remaut, and G. Waksman, Architectures and biogenesis of non-flagellar protein appendages in Gram-negative bacteria, EMBO J, vol.27, pp.2271-2280, 2008.

F. X. Gomis-ruth and M. Coll, Cut and move: protein machinery for DNA processing in bacterial conjugation, Curr Opin Struct Biol, vol.16, pp.744-752, 2006.

R. Bayliss, NMR structure of a complex between the VirB9/VirB7 interaction domains of the pKM101 type IV secretion system, Proc Natl Acad Sci U S A, vol.104, pp.1673-1678, 2007.

R. Fronzes, Structure of a type IV secretion system core complex, Science, vol.323, pp.266-268, 2009.

S. J. Jakubowski, Agrobacterium VirB10 domain requirements for type IV secretion and T pilus biogenesis, Mol Microbiol, vol.71, pp.779-794, 2009.

. Chandran, , vol.8

, Author manuscript; available in PMC, Nature, 2010.

P. Europe and L. Funders-;-terradot, Structures of two core subunits of the bacterial type IV secretion system, VirB8 from Brucella suis and ComB10 from Helicobacter pylori, Proc Natl Acad Sci U S A, vol.102, pp.4596-4601, 2005.

C. Dong, Wza the translocon for E. coli capsular polysaccharides defines a new class of membrane protein, Nature, vol.444, pp.226-229, 2006.

G. Meng, R. Fronzes, V. Chandran, H. Remaut, and G. Waksman, Protein oligomerization in the bacterial outer membrane (Review), Mol Membr Biol, vol.26, pp.136-145, 2009.

V. Koronakis, A. Sharff, E. Koronakis, B. Luisi, and C. Hughes, Crystal structure of the bacterial membrane protein TolC central to multidrug efflux and protein export, Nature, vol.405, pp.914-919, 2000.

E. Cascales and P. J. Christie, Agrobacterium VirB10, an ATP energy sensor required for type IV secretion, Proc Natl Acad Sci U S A, vol.101, pp.17228-17233, 2004.

K. Atmakuri, E. Cascales, and P. J. Christie, Energetic components VirD4, VirB11 and VirB4 mediate early DNA transfer reactions required for bacterial type IV secretion, Mol Microbiol, vol.54, pp.1199-1211, 2004.

M. Llosa, S. Zunzunegui, and F. De-la-cruz, Conjugative coupling proteins interact with cognate and heterologous VirB10-like proteins while exhibiting specificity for cognate relaxosomes, Proc Natl Acad Sci U S A, vol.100, pp.10465-10470, 2003.

E. Cascales and P. J. Christie, Definition of a bacterial type IV secretion pathway for a DNA substrate, Science, vol.304, pp.1170-1173, 2004.

A. J. Sharff, E. Koronakis, B. Luisi, and V. Koronakis, Oxidation of selenomethionine: some MADness in the method!, Acta Crystallogr D Biological Crystallography, vol.56, pp.785-788, 2000.

W. Kabsch, Automatic processing of rotation diffraction data from crystals of initially unknown symmetry and cell constants, Journal of applied crystallography, vol.26, pp.795-800, 1993.

J. Navaza, AMoRe: an automated package for molecular replacement, Acta Crystallographica Section A: Foundations of Crystallography, vol.50, pp.157-163, 1994.

T. A. Jones, A set of averaging programs, Molecular Replacement". CCP4 Proceedings, pp.91-95, 1992.

M. Kjeldgaard and T. A. Jones, From First Map to Final Model, CCP4 Proceedings, pp.59-66, 1994.

G. Kleywegt and T. Jones, Software for handling macromolecular envelopes, Acta Crystallographica Section D: Biological Crystallography, vol.55, pp.941-944, 1999.

, Collaborative Computational Project, N. The CCP4 suite: programs for protein crystallography, Acta Crystallogr D Biological Crystallography, vol.50, pp.760-763, 1994.

P. Emsley and K. Cowtan, Coot: model-building tools for molecular graphics, Acta Crystallogr D Biological Crystallography, vol.60, pp.2126-2132, 2004.

A. Mccoy, Phaser crystallographic software, Journal of applied crystallography, vol.40, pp.658-674, 2007.

G. Murshudov, A. Vagin, and E. Dodson, Refinement of macromolecular structures by the maximumlikelihood method, Acta Crystallographica Section D: Biological Crystallography, vol.53, pp.240-255, 1997.

P. D. Adams, PHENIX: building new software for automated crystallographic structure determination, Acta Crystallogr D Biological Crystallography, vol.58, pp.1948-1954, 2002.

P. V. Afonine, R. W. Grosse-kunstleve, and P. D. Adams, The Phenix refinement framework. CCP4 Newsletter, p.42, 2005.