Périgord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis - Archive ouverte HAL Access content directly
Journal Articles Nature Year : 2010

Périgord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis

(1) , (1) , (1) , (2) , (3) , (4) , (5) , (1, 6) , (4) , (5) , (4) , (7) , (8) , (9) , (4, 10) , (7) , (4, 10) , (4, 10) , (11) , (5) , (12) , (1) , (1) , (3) , (13) , (4) , (4, 10) , (4, 10) , (1) , (2) , (1) , (14) , (1) , (15) , (16) , (17) , (18) , (19) , (5) , (20) , (8) , (1) , (5) , (14) , (21) , (22) , (23) , (24) , (21) , (5) , (4)
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
Annegret Kohler
Claude Murat
Sergio Arcioni
  • Function : Author
  • PersonId : 948046
Annick Brun
  • Function : Author
  • PersonId : 770120
  • IdRef : 191648620
Marc Buée
Benoit Marçais
Hadi Quesneville
Claudia Riccioni
  • Function : Author
Emilie Tisserant
  • Function : Author
  • PersonId : 770121
  • IdRef : 159351952

Abstract

The Périgord black truffle ($Tuber\ melanosporum$ Vittad.) and the Piedmont white truffle dominate today's truffle market. The hypogeous fruiting body of $T.\ melanosporum$ is a gastronomic delicacy produced by an ectomycorrhizal symbiont endemic to calcareous soils in southern Europe. The worldwide demand for this truffle has fuelled intense efforts at cultivation. Identification of processes that condition and trigger fruit body and symbiosis formation, ultimately leading to efficient crop production, will be facilitated by a thorough analysis of truffle genomic traits. In the ectomycorrhizal $Laccaria\ bicolor$, the expansion of gene families may have acted as a 'symbiosis toolbox'. This feature may however reflect evolution of this particular taxon and not a general trait shared by all ectomycorrhizal species. To get a better understanding of the biology and evolution of the ectomycorrhizal symbiosis, we report here the sequence of the haploid genome of $T.\ melanosporum$, which at $\sim$125 megabases is the largest and most complex fungal genome sequenced so far. This expansion results from a proliferation of transposable elements accounting for $\sim$58% of the genome. In contrast, this genome only contains $\sim$7,500 protein-coding genes with very rare multigene families. It lacks large sets of carbohydrate cleaving enzymes, but a few of them involved in degradation of plant cell walls are induced in symbiotic tissues. The latter feature and the upregulation of genes encoding for lipases and multicopper oxidases suggest that $T.\ melanosporum$ degrades its host cell walls during colonization. Symbiosis induces an increased expression of carbohydrate and amino acid transporters in both $L.\ bicolor$ and $T.\ melanosporum$, but the comparison of genomic traits in the two ectomycorrhizal fungi showed that genetic predispositions for symbiosis $-$'the symbiosis toolbox'$-$ evolved along different ways in ascomycetes and basidiomycetes
Fichier principal
Vignette du fichier
mar.pdf (922.41 Ko) Télécharger le fichier
Origin : Publication funded by an institution
Loading...

Dates and versions

cea-00907731 , version 1 (17-10-2019)

Identifiers

Cite

Francis Martin, Annegret Kohler, Claude Murat, Raffaella Balestrini, Pedro M Coutinho, et al.. Périgord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis. Nature, 2010, 464 (7291), pp.1033-1038. ⟨10.1038/nature08867⟩. ⟨cea-00907731⟩
866 View
75 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More