Evaluation of High Resolution Magic-Angle Coil Spinning NMR Spectroscopy for Metabolic Profiling of Nanoliter Tissue Biopsies - Archive ouverte HAL Access content directly
Journal Articles Analytical Chemistry Year : 2012

Evaluation of High Resolution Magic-Angle Coil Spinning NMR Spectroscopy for Metabolic Profiling of Nanoliter Tissue Biopsies

(1) , (2) , (1) , (2) , (2) , (2) , (1)
1
2

Abstract

High-resolution magic-angle sample spinning (HR-MAS) 1H NMR spectroscopy of tissue biopsies combined with chemometric techniques has emerged as a valuable methodology for disease diagnosis and environmental assessments. However, the tissue mass required for such experiments is of the order of 10 mg, and this can compromise the metabolic evaluation because of tissue heterogeneity. Tissue availability is often a limitation for clinical studies due to histopathological requirements, which are currently the gold standard for diagnosis, for example, in the case of tumors. Here, we introduce the use of a rotating micro-NMR detector that optimizes the coil filling factor such that mass-limited samples can be measured. We show the results for measuring nanoliter volume tissue biopsies using a commercial HR-MAS probe for the first time. The method has been tested with bovine muscle and human gastric mucosal tumor tissue samples. The gain in mass sensitivity is approximate up to 17-fold, and the adequate spectral resolution (3 Hz) allows the measurement of the metabolite profiles in nanoliter volume samples, thereby limiting the ambiguity resulting from heterogeneous tissues; thus, the approach presents diagnostic potential for studies by metabonomics of mass-limited biopsies.
Not file

Dates and versions

cea-00853774 , version 1 (23-08-2013)

Identifiers

Cite

Alan Wong, Beatriz Jiménez, Xiaonan Li, Elaine Holmes, Jeremy K. Nicholson, et al.. Evaluation of High Resolution Magic-Angle Coil Spinning NMR Spectroscopy for Metabolic Profiling of Nanoliter Tissue Biopsies. Analytical Chemistry, 2012, 84, pp.3843 - 3848. ⟨10.1021/ac300153k⟩. ⟨cea-00853774⟩

Collections

CEA IRAMIS
157 View
0 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More