AUX/LAX genes encode a family of auxin influx transporters that perform distinct functions during Arabidopsis development. - Archive ouverte HAL Access content directly
Journal Articles The Plant cell Year : 2012

AUX/LAX genes encode a family of auxin influx transporters that perform distinct functions during Arabidopsis development.

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Kamal Swarup
  • Function : Author
Alison Ferguson
  • Function : Author
Malvika Seth
  • Function : Author
Yaodong Yang
  • Function : Author
Stijn Dhondt
  • Function : Author
Nicholas James
  • Function : Author
Paula Perry
  • Function : Author
Adnan Syed
  • Function : Author
Haibing Yang
  • Function : Author
Jesica Reemmer
  • Function : Author
Edward Venison
  • Function : Author
Caroline Howells
  • Function : Author
Miguel A Perez-Amador
  • Function : Author
Jeonga Yun
  • Function : Author
Jose Alonso
  • Function : Author
Ranjan Swarup
  • Function : Author

Abstract

Auxin transport, which is mediated by specialized influx and efflux carriers, plays a major role in many aspects of plant growth and development. AUXIN1 (AUX1) has been demonstrated to encode a high-affinity auxin influx carrier. In Arabidopsis thaliana, AUX1 belongs to a small multigene family comprising four highly conserved genes (i.e., AUX1 and LIKE AUX1 [LAX] genes LAX1, LAX2, and LAX3). We report that all four members of this AUX/LAX family display auxin uptake functions. Despite the conservation of their biochemical function, AUX1, LAX1, and LAX3 have been described to regulate distinct auxin-dependent developmental processes. Here, we report that LAX2 regulates vascular patterning in cotyledons. We also describe how regulatory and coding sequences of AUX/LAX genes have undergone subfunctionalization based on their distinct patterns of spatial expression and the inability of LAX sequences to rescue aux1 mutant phenotypes, respectively. Despite their high sequence similarity at the protein level, transgenic studies reveal that LAX proteins are not correctly targeted in the AUX1 expression domain. Domain swapping studies suggest that the N-terminal half of AUX1 is essential for correct LAX localization. We conclude that Arabidopsis AUX/LAX genes encode a family of auxin influx transporters that perform distinct developmental functions and have evolved distinct regulatory mechanisms.

Dates and versions

cea-00848564 , version 1 (26-07-2013)

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Benjamin Péret, Kamal Swarup, Alison Ferguson, Malvika Seth, Yaodong Yang, et al.. AUX/LAX genes encode a family of auxin influx transporters that perform distinct functions during Arabidopsis development.. The Plant cell, 2012, 24 (7), pp.2874-85. ⟨10.1105/tpc.112.097766⟩. ⟨cea-00848564⟩
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