 |
Journal articles
Dynamic study of blood-brain barrier closure after its disruption using ultrasound: a quantitative analysis.
Abstract : Delivery of therapeutic or diagnostic agents to the brain is majorly hindered by the blood-brain barrier (BBB). Recently, many studies have demonstrated local and transient disruption of the BBB using low power ultrasound sonication combined with intravascular microbubbles. However, BBB opening and closure mechanisms are poorly understood, especially the maximum gap that may be safely generated between endothelial cells and the duration of opening of the BBB. Here, we studied BBB opening and closure under magnetic resonance (MR) guidance in a rat model. First, MR contrast agents (CA) of different hydrodynamic diameters (1 to 65 nm) were employed to estimate the largest molecular size permissible across the cerebral tissues. Second, to estimate the duration of the BBB opening, the CA were injected at various times post-BBB disruption (12 minutes to 24 hours). A T(1) mapping strategy was developed to assess CA concentration at the ultrasound (US) focal point. Based on our experimental data and BBB closure modeling, a calibration curve was obtained to compute the half closure time as a function of CA hydrodynamic diameter. These findings and the model provide an invaluable basis for optimal design and delivery of nanoparticles to the brain.
Cited literature [39 references]
https://hal-cea.archives-ouvertes.fr/cea-00739117
Contributor : Sébastien Mériaux Connect in order to contact the contributor
Submitted on : Friday, October 5, 2012 - 5:35:18 PM
Last modification on : Tuesday, September 28, 2021 - 5:13:17 PM
Long-term archiving on: : Sunday, January 6, 2013 - 3:52:29 AM
Contributor : Sébastien Mériaux Connect in order to contact the contributor
Submitted on : Friday, October 5, 2012 - 5:35:18 PM
Last modification on : Tuesday, September 28, 2021 - 5:13:17 PM
Long-term archiving on: : Sunday, January 6, 2013 - 3:52:29 AM
File
Marty_JCBFM_proof.pdf
Files produced by the author(s)