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Stimulation of pyrene mineralization in freshwater sediments by bacterial and plant bioaugmentation

Abstract : As a means to study the fate of PAHs in freshwater sediments, pyrene mineralization was examined in microcosms spiked with 14C-pyrene. Some microcosms were planted with reeds (Phragmites australis) and/or inoculated with a pyrene-degrading strain, Mycobacterium sp. 6PY1. Mineralization rates recorded over a 61-d period showed that reeds promoted a significant enhancement of pyrene degradation, which possibly resulted from a root-mediated increase of oxygen diffusion into the sediment layer, as indicated by in situ redox measurements. In inoculated microcosms, mineralization reached a higher level in the absence (8.8%) than in the presence of plants (4.4%). Mineralization activity was accompanied by the release of water-soluble pyrene oxidation products, the most abundant of which was identified as 4,5-diphenanthroic acid. Pyrene was recovered from plant tissues, including stems and leaves, at concentrations ranging between 40 and 240µg/g dry weight. Plants also accumulated labeled oxidation products likely derived from microbial degradation. Pyrene-degrading strains were 35-70 fold more abundant in inoculated than in non-inoculated microcosms. Most of the pyrene-degrading isolates selected from the indigenous microflora, were identified as M. austroafricanum strains. Taken together, the results of this study show that plants or PAH-degrading bacteria enhance pollutant removal, but their effects are not necessarily cumulative.
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Contributor : Yves Jouanneau Connect in order to contact the contributor
Submitted on : Friday, April 24, 2009 - 9:44:49 AM
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Yves Jouanneau, J. C. Willison, Christine Meyer, S. Krivobok, N. Chevron, et al.. Stimulation of pyrene mineralization in freshwater sediments by bacterial and plant bioaugmentation. Environmental Science and Technology, American Chemical Society, 2005, 39, pp.5229-5235. ⟨10.1021/es050412d⟩. ⟨hal-00378341⟩



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