C-fos and c-myc modulation, mitogenic effect and Ia expression in the P388D1 murine macrophage line treated by immunomodifiers.

Magali Le Discorde (1) , Y. C. Le Garrec (1) , E. H. Jullian (1) , P. J. Michel (1) , A. J. Pompidou (1)

1 Laboratoire Histologie, Cytogénétique et Institut de Pathologie Moléculaire

The aim of the present study was to investigate whether the early modulation of the c-fos and c-myc oncogenes could give some orientation to the impact of immunomodulators on the monocyte-macrophage lineage. In order to work in a homogeneous system we used the P388D1 mouse macrophage cell-line which is considered as an almost mature macrophage. When P388D1 cells were stimulated by LPS, interferon-gamma or the association of both compounds, no direct correlation could be found between the modulation of DNA synthesis and the early expression of the c-fos and c-myc oncogenes. The positive regulation of Ia antigen expression seemed to correlate with the absence of induction of c-fos oncogene.

Domaines: Biochimie, Biologie Moléculaire

Magali Le Discorde, Y. C. Le Garrec, E. H. Jullian, P. J. Michel, A. J. Pompidou. C-fos and c-myc modulation, mitogenic effect and Ia expression in the P388D1 murine macrophage line treated by immunomodifiers.. Dev Biol Stand, 1992, 77, pp.65-70. ⟨cea-00274272⟩