Native Collagen: Electrospinning of Pure, Cross-Linker-Free, Self-Supported Membrane

Dounia Dems (1) , Julien Rodrigues da Silva (1) , Christophe Hélary (1) , Frank Wien (2) , Marion Marchand , Nicolas Debons (1) , Laurent Muller (3) , Yong Chen , Marie-Claire Schanne-Klein (4) , Christel Laberty-Robert (5) , Natacha Krins (5) , Carole Aimé (1)

1 LCMCP-MATBIO - Matériaux et Biologie
2 SSOLEIL - Synchrotron SOLEIL
3 CIRB - Centre interdisciplinaire de recherche en biologie
4 LOB - Laboratoire d'Optique et Biosciences
5 LCMCP-RMES - Reactive Materials for Electrochemical Systems

Rebuilding biological environments is crucial when facing the challenges of fundamental and biomedical research. Thus, preserving the native state of biomolecules is essential. We use electrospinning (ES), which is an extremely promising method for the preparation of fibrillar membranes to mimic the ECM of native tissues. Here, we report for the first time (1) the ES of pure and native collagen into a self-supported membrane in absence of crosslinker and polymer support, (2) the preservation of the membrane integrity in hydrated media in absence of crosslinker and (3) the preservation of the native molecular structure and recovery of the hierarchical assembly of collagen. We use a multiscale approach to characterize collagen native structure at the molecular level using circular dichroism, and to investigate collagen hierarchical organization within the self-supported membrane using a combination of multiphoton and electron microscopies. Finally, we show that the membranes are perfectly suited for cell adhesion and spreading, making them very promising candidates for the development of biomaterials and finding applications in biomedical research.

Mots clés: Collagen; electrospinning; hierarchical organization; extra-cellular matrix; tissue engineering; second harmonic generation microscopy

Domaines: Matériaux

Dounia Dems, Julien Rodrigues da Silva, Christophe Hélary, Frank Wien, Marion Marchand, et al.. Native Collagen: Electrospinning of Pure, Cross-Linker-Free, Self-Supported Membrane. ACS Applied Bio Materials, 2020, ⟨10.1021/acsabm.0c00006⟩. ⟨hal-02541707⟩