Rates of Chemical Reactions Embedded in a Metabolic Network by Dissolution Dynamic Nuclear Polarisation NMR

Aude Sadet (1) , Emmanuelle M. M. Weber (1) , Aditya Jhajharia (1) , Dennis Kurzbach (2, 1) , Geoffrey Bodenhausen (1) , Emeric Miclet (1) , Daniel Abergel (3)

1 LBM UMR 7203 - Laboratoire des biomolécules
2 LBM-E3 - Structure et Dynamique des Biomolécules
3 LBM UMR 7203 - Laboratoire des biomolécules

The isomerisation of 6-phosphogluconolactones and their hydrolyses into 6-phosphogluconic acid form a non enzymatic side cycle of the pentose-phosphate pathway (PPP) in cells. We show that dissolution dynamic nuclear polarization can be used for determining the kinetic rates of the involved transformations in real time. It is found that the hydrolysis of both lactones is significantly slower than the isomeration process, thereby shedding new light onto this subtle chemical process.

Domains: Chemical Sciences

Aude Sadet, Emmanuelle M. M. Weber, Aditya Jhajharia, Dennis Kurzbach, Geoffrey Bodenhausen, et al.. Rates of Chemical Reactions Embedded in a Metabolic Network by Dissolution Dynamic Nuclear Polarisation NMR. Chemistry - A European Journal, 2018, 24 (21), pp.5456-5461. ⟨10.1002/chem.201705520⟩. ⟨hal-02352047⟩