Controlled E. coli aggregation mediated by DNA and XNA hybridization. Authors - Institut de Biologie François JACOB Accéder directement au contenu
Article Dans Une Revue ChemBioChem Année : 2023

Controlled E. coli aggregation mediated by DNA and XNA hybridization. Authors

Résumé

Chemical cell surface modification is a fast-growing field of research, due to its enormous potential in tissue engineering, cell-based immunotherapy, and regenerative medicine. However, engineering of bacterial tissues by chemical cell surface modification has been vastly underexplored and the identification of suitable molecular handles is in dire need. We present here, an orthogonal nucleic acid-protein conjugation strategy to promote artificial bacterial aggregation. This system gathers the high selectivity and stability of linkage to a protein Tag expressed at the cell surface and the modularity and reversibility of aggregation due to oligonucleotide hybridization. For the first time, XNA (xeno nucleic acids in the form of 1,5-anhydrohexitol nucleic acids) were immobilized via covalent, SNAP-tag-mediated interactions on cell surfaces to induce bacterial aggregation.

Domaines

Chimie
Fichier principal
Vignette du fichier
MS snap-odn_V3 revised_MH.pdf (1.37 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
licence : CC BY NC - Paternité - Pas d'utilisation commerciale

Dates et versions

pasteur-04104867 , version 1 (24-05-2023)

Licence

Paternité - Pas d'utilisation commerciale

Identifiants

Citer

Cécile Gasse, Puneet Srivastava, Guy Shepers, Joachim Jose, Marcel Hollenstein, et al.. Controlled E. coli aggregation mediated by DNA and XNA hybridization. Authors. ChemBioChem, inPress, pp.e202300191. ⟨10.1002/cbic.202300191⟩. ⟨pasteur-04104867⟩
37 Consultations
52 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More